Facilities & Resources

Within our group, we have the tools and expertise to perform research in the following focus areas: chemical separations and mass spectrometry, synthesis, and bioanalytical chemistry, particularly in the development of nucleic acid extraction methodolgies. We occupy approximately 3,000 square feet of combined laboratory and office space in Gilman Hall and Hach Hall. All synthesis and biological research is performed in the Gilman Hall lab while all chromatography and mass spectrometry research is carried out in the newly constructed, custom-designed Hach Hall labspace.

Instrumentation within our laboratory for chemical separations and mass spectrometry includes:

  • Two (2) Shimadzu Prominence HPLCs employing variable wavelength and fluorescence detectors
  • Two (2) Agilent Technologies 1260 HPLCs employing photodiode array detectors
  • Agilent Technologies 1260 Infinity II HPLC employing photodiode array detection
  • Agilent Technologies 6230B accurate mass TOF LC/MS coupled to a 1260 HPLC with a phototodiode array detector
  • Two (2) Agilent Technologies 7890/5977 GC/MS systems
  • Agilent Technologies 7697A Headspace Sampler
  • Two (2) Agilent Technologies 6850 GCs
  • A homebuilt comprehensive GCxGC system employing a cryogenic modulator built from an Agilent Technologies 6890 GC
  • Gerstel Technologies thermal desorption unit (TDU)
  • Two (2) Agilent Technologies 7890 GCs employing flame ionization, thermal conductivity, and electron capture detection

    Within our lab, we have two water bath/vacuum assembly stations designed for the static coating of GC capillary columns. We also have all necessary tools for the high-throughput production of SPME extraction devices using spin coating within a Rayonet photochemical UV reactor employing a rotating carousel. The laboratory also has two (2) analytical balances, two (2) top loading balances, and a Mettler-Toledo microbalance.

Tools within our laboratory for synthesis and characterization includes:

  • Heating mantles
  • Round bottom flasks
  • Dean-Stark traps for azeotropic distillation
  • Separatory funnels
  • Reflux condensers
  • Two (2) vacuum ovens
  • UV reactors for "Click" chemistry
  • Two (2) Buchi rotary evaporators
  • UV lamps for TLC plates
  • TLC developing chambers
  • Metrohm coulometric Karl Fischer titrator
  • Du Nouy ring surface tensiometer
  • Brookfield Engineering cone and plate viscometer
  • Ultimaker 3 Model 3D Printer for the production of microextraction devices and components

    We use shared instrumentation in the Chemical Instrumentation Facility located in the chemistry department. This includes 600 and 400 MHz NMR spectrometers, FT-IR and UV-Vis spectrometers, DSC/TGA, and CHN analyzer. Characterization of the paramagnetic properties for the magnetic ionic liquids synthesized in our group is performed using a SQUID magnetometer in collaboration with scientists from Ames Laboratory on campus.

Instrumentation and supplies within our laboratory for bioanalytical and nucleic acid chemistry include:

  • NuAire Class II, Type A2 biological safety cabinet
  • Two (2) Eppendorf incubator shakers
  • Two (2) Microcentrifuges
  • Benchtop centrifuge
  • Eppendorf 5920R refrigerated centrifgue
  • Fisher Scientific Isotemp dual chamber thermostatted water bath
  • Thermolyne benchtop incubator
  • Corning low speed orbital shaker
  • Revco -86°C freezer
  • Revco -25°C freezer
  • Thermo Scientific NanoDrop 2000c spectrophotometer
  • BioTek Synergy Hybrid H1 Microplate Reader with Take3 Micro-Volume Plate
  • Bio Rad CFX96 TouchTM Real-Time PCR System
  • Bio Rad CFX ConnectTM Real-Time PCR System
  • Techne FTgene2D thermal cycler for PCR
  • Techne 3PRIMEBASE thermal cycler for PCR
  • Bio Rad Gene Pulser Xcell electroporator with PC and CE modules
  • Bethesda Research Laboratories H4 horizontal gel electrophoresis system with a Neo/Sci dual output power supply
  • Thermo Scientific Safe Imager Blue Light Transilluminator
  • Qiagen miniprep kits for nucleic acid extraction

    The synthesis and sequencing of oligonucleotides for our group's work can be done at the ISU DNA Facility located across the street from our laboratory.